Extracellular polymeric substance(EPS) in biological wastewater treatment system,incarnating the function and composition of microorganisms,would contribute obviously to sludge characteristics and the performance of the system.However,standard method for measuring EPS has been not determined because of its numerous influencing factors.Therefore,the factor of EPS was analyzed synthetically in this paper,such as the influent components and operating conditions,which including sludge loading,dissolved oxygen and hydraulic detention time.Different extracting mode and measuring method were also comparatively analyzed.It would accelerate the control effect on the treatment efficiency of biological treatment system by further study on EPS and its function