生物分子
化学
离子色谱法
选择性
离子交换
亲和层析
DNA
色谱法
柱色谱法
组合化学
生物化学
有机化学
离子
酶
催化作用
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 179-192
标识
DOI:10.1007/978-1-0716-3362-5_10
摘要
Hydroxyapatite (HA) is a mixed-mode media that has been used extensively for the purification of proteins and DNA since the 1950s. Hydroxyapatite possesses a distinctive selectivity that may be applied in the purification of a wide range of biomolecules: immunoglobulins, alkaline proteins, acidic proteins, and DNA. The functional groups of HA can both attract and repel the carboxyl and amino groups on target molecules. This unique selectivity is due to the modalities that can be employed, which are not possible with traditional anion-exchange and cation-exchange chromatography. HA is a powerful chromatography step for reducing host cell-derived impurities and aggregated product, where a 2-4 log reduction in host cell proteins, aggregates, endotoxin, and viruses are routinely achieved. This chapter describes the procedures for: efficiently packing and evaluating a HA column, purifying IgG and acidic proteins respectively using HA chromatography.
科研通智能强力驱动
Strongly Powered by AbleSci AI