神经炎症
小胶质细胞
背景(考古学)
促炎细胞因子
大麻素受体2型
刺激
脂多糖
神经科学
炎症
信使核糖核酸
电池类型
受体
生物
细胞
医学
细胞生物学
大麻素受体
免疫学
内科学
兴奋剂
基因
古生物学
生物化学
遗传学
作者
Wanda Grabon,Anne Ruiz,Nadia Gasmi,Cyril Dégletagne,Béatrice Georges,Amor Belmeguenaï,Jacques Bodennec,Sylvain Rheims,Guillaume Marcy,Laurent Bezin
出处
期刊:Research Square - Research Square
日期:2024-01-30
标识
DOI:10.21203/rs.3.rs-3870149/v1
摘要
Abstract Since its detection in the brain, the cannabinoid receptor type 2 (CB2) has been considered a promising therapeutic target for various neurological and psychiatric disorders. However, precise brain mapping of its expression is still lacking. Using magnetic cell sorting, calibrated RT-qPCR and single-nucleus RNAseq, we show that CB2 is expressed at a low level in all brain regions studied, mainly by few microglial cells, and by neurons in an even lower proportion. Upon lipopolysaccharide stimulation, modeling neuroinflammation in non-sterile conditions, we demonstrate that the inflammatory response is associated with a transient reduction in CB2 mRNA levels in brain tissue, particularly in microglial cells. This result, confirmed in the BV2 microglial cell line, contrasts with the positive correlation observed between CB2 mRNA levels and the inflammatory response upon stimulation by interferon-gamma, modeling neuroinflammation in sterile condition. Discrete brain CB2 expression might thus be up- or down-regulated depending on the inflammatory context.
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