Evaluation of the immobilized enzymes function in soil remediation following polycyclic aromatic hydrocarbon contamination

The bioremediation of polycyclic aromatic hydrocarbon (PAHs) from soil utilizing microorganisms, enzymes, microbial consortiums, strains, etc. has attracted a lot of interest due to the environmentally friendly, and cost-effective features. Enzymes can efficiently break down PAHs in soil by hydroxylating the benzene ring, breaking the C-C bond, and catalyze the hydroxylation of a variety of benzene ring compounds via single-electron transfer oxidation. However, the practical application is limited by its instability and ease to loss function under harsh environmental conditions such as pH, temperature, and edaphic stress etc. Therefore, this paper focused on the techniques used to immobilize enzymes and remediate PAHs in soil. Moreover, previous research has not adequately covered this topic, despite the employment of several immobilized enzymes in aqueous solution cultures to remediate other types of organic pollutants. Bibliometric analysis further highlighted the research trends from 2000 to 2023 on this field of growing interest and identified important challenges regarding enzyme stability and interaction with soil matrices. The findings indicated that immobilized enzymes may catalyzed PAHs via oxidation of OH groups in benzene rings, and generate benzyl radicals (i.e.,