恒河猴
嗜酸性粒细胞
离体
嗜酸性粒细胞增多症
生物
嗜酸性粒细胞过氧化物酶
猕猴
免疫学
外周血
体外
医学
哮喘
神经科学
生物化学
出处
期刊:Methods in molecular biology
日期:2021-01-01
标识
DOI:10.1007/978-1-0716-1095-4_3
摘要
Eosinophils are granulocytes involved mainly in allergic inflammation and parasitic responses and constitute 1-5% of the circulating leukocytes in human healthy subjects. New immunotherapies targeting eosinophils have been developed and evaluated recently, and the availability of animal models that could mimic human eosinophil responses is important to consider. Differences in eosinophil biology and pathogenesis between humans and murine models have limited their utility in some settings. Isolation of viable eosinophils from rhesus macaque blood suitable for ex vivo and in vitro experimentation could provide a valuable tool for the study of eosinophil-targeted therapies and for the exploration of eosinophilic associated responses. Here, a new technique for the isolation of human eosinophils from rhesus macaque blood by negative selection from whole blood is described.
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