脱氧核酶
化学
生物物理学
胶体金
DNA
小RNA
纳米技术
纳米颗粒
生物化学
基因
生物
材料科学
作者
Yanjing Yang,Jin Huang,Xiaohai Yang,Xiaoxiao He,Ke Quan,Nuli Xie,Min Ou,Kemin Wang
标识
DOI:10.1021/acs.analchem.7b00174
摘要
A new class of intracellular nanoprobe, termed AuNP-based hairpin-locked-DNAzyme probe, was developed to sense miRNA in living cells. Briefly, it consists of an AuNP and hairpin-locked-DNAzyme strands. In the absence of target miRNA, the hairpin-locked-DNAzyme strand forms a hairpin structure by intramolecular hybridization, which could inhibit the catalytic activity of DNAzyme strand and the fluorescence is quenched by the AuNP. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active secondary structure in the catalytic cores to yield an "active" DNAzyme, which then cleaves the self-strand with the assist of Mg2+. The cleaved two shorter DNA fragments are separated with the target. As a result, the fluorophores are released from the AuNP and the fluorescence is enhanced. Meanwhile, the target is also released and binds to another hairpin-locked-DNAzyme strand to drive another cycle of activation. In such a way, the target-recycling amplification leads to significant signal enhancement and thus offers high detection sensitivity.
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