The New Strategy of Breeding Cytidine Excessive Biosynthesis Mutants by pyr Operon Rearrangement of Bacillus amyloliquefaciens

Cytidine is a good antitumor and antiviral intermediate which can also be used as a healthy food ingredients. With the market for cytidine increasing, the large-scale production of cytidine by microbial fermentation method has become a major way to solve this problem. While improving cytidine production, the regulation of pyr operon is essential to the excessive synthesis cytidine. However, its transcriptional regulation mechanism is unclear. The passage summarizes the regulation of de novo pyrimidine nucleotide biosynthesis (pyr genes) and the metabolic regulation mechanism of cytidine synthesis in Bacillus amyloliquefaciens. This paper makes the regulation protein pyrR as the research object, presents a series of Bacillus amyloliquefaciens pyrimidine operon transcriptional regulatory rearrangement strategy, effects the transcriptional regulation on cytidine biosynthesis, and aims to provide a theoretical basis for cytidine yielding strain.