Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21

内生菌 生物 枯草芽孢杆菌 植物 假单胞菌 生物病虫害防治 园艺 细菌 遗传学
作者
Shahzad Munir,Yongmei Li,Pengbo He,Pengfei He,Pengjie He,Wenyan Cui,Yixin Wu,Xingyu Li,Li Qi,Zhang Sixiang,Yangsu Xiong,Zhanjun Lu,Wenbiao Wang,Kexian Zong,Yongchao Yang,Shaocong Yang,Chan Mu,Heming Wen,Yue‐Hu Wang,Jun Guo,Samantha C. Karunarathna,Yueqiu He
出处
期刊:Frontiers in Plant Science [Frontiers Media SA]
卷期号:12 被引量:11
标识
DOI:10.3389/fpls.2021.789065
摘要

Huanglongbing (HLB) has turned into a devastating botanical pandemic of citrus crops, caused by Candidatus Liberibacter asiaticus (CLas). However, until now the disease has remained incurable with very limited control strategies available. Restoration of the affected microbiomes in the diseased host through the introduction of an indigenous endophyte Bacillus subtilis L1-21 isolated from healthy citrus may provide an innovative approach for disease management. A novel half-leaf method was developed in vitro to test the efficacy of the endophyte L1-21 against CLas. Application of B. subtilis L1-21 at 104 colony forming unit (cfu ml-1) resulted in a 1,000-fold reduction in the CLas copies per gram of leaf midrib (107 to 104) in 4 days. In HLB-affected citrus orchards over a period of 2 years, the CLas incidence was reduced to < 3%, and CLas copies declined from 109 to 104 g-1 of diseased leaf midribs in the endophyte L1-21 treated trees. Reduction in disease incidence may corroborate a direct or an indirect biocontrol effect of the endophytes as red fluorescent protein-labeled B. subtilis L1-21 colonized and shared niche (phloem) with CLas. This is the first large-scale study for establishing a sustainable HLB control strategy through citrus endophytic microbiome restructuring using an indigenous endophyte.
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