A new salamo-based probe H2L was synthesized and characterized by nuclear magnetic resonance, mass spectrometry and fluorescence spectroscopy. Compared with previous work of some other probes, the probe H2L can selectively recognize Al3+ in the fluorescence spectra and the detection limit is as low as 5.98 × 10−9 M. Beyond that, the color of solution becomes bright green under 365 nm UV light. Notably, under low concentrations, the probe can be used as a tool for exogenous detection of Al3+ in organisms. This result expands the application of salamo-based fluorescent probes in the field of biology.