巨噬细胞
库普弗电池
单核细胞
病理
免疫荧光
免疫组织化学
生物
门静脉
肝细胞
免疫学
医学
体外
抗体
内科学
生物化学
作者
Adrien Guillot,Chloé Buch,Tony Jourdan
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 45-53
被引量:7
标识
DOI:10.1007/978-1-0716-0704-6_6
摘要
Kupffer cells are the liver-resident macrophages and represent the first line of defense between the pathogens circulating from the intestines through the portal vein and systemic circulation. Recent works have highlighted the complex heterogeneity of macrophage functions and origins, thus raising awareness on the need for a better characterization of macrophage populations. The immunohistochemistry method here described, allows for a rapid distinction between Kupffer cells and monocyte-derived macrophages present on formalin-fixed, paraffin-embedded mouse liver samples. This protocol has been optimized for its reproducibility, reliability, and simplicity.
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