突触后电位
生物
神经传递
神经科学
细胞生物学
带状突触
细胞外基质
受体
突触小泡
生物化学
膜
小泡
作者
Dipak N. Patil,Serena Pantalone,Yan Cao,Thibaut Laboute,Scott J. Novick,Shikha Singh,Simone Savino,Silvia Faravelli,Francesca Magnani,Patrick R. Griffin,Appu K. Singh,Federico Forneris,Kirill A. Martemyanov
出处
期刊:Science Signaling
[American Association for the Advancement of Science (AAAS)]
日期:2023-07-25
卷期号:16 (795)
被引量:5
标识
DOI:10.1126/scisignal.add9539
摘要
Precise synapse formation is essential for normal functioning of the nervous system. Retinal photoreceptors establish selective contacts with bipolar cells, aligning the neurotransmitter release apparatus with postsynaptic signaling cascades. This involves transsynaptic assembly between the dystroglycan-dystrophin complex on the photoreceptor and the orphan receptor GPR179 on the bipolar cell, which is mediated by the extracellular matrix protein pikachurin (also known as EGFLAM). This complex plays a critical role in the synaptic organization of photoreceptors and signal transmission, and mutations affecting its components cause blinding disorders in humans. Here, we investigated the structural organization and molecular mechanisms by which pikachurin orchestrates transsynaptic assembly and solved structures of the human pikachurin domains by x-ray crystallography and of the GPR179-pikachurin complex by single-particle, cryo–electron microscopy. The structures reveal molecular recognition principles of pikachurin by the Cache domains of GPR179 and show how the interaction is involved in the transsynaptic alignment of the signaling machinery. Together, these data provide a structural basis for understanding the synaptic organization of photoreceptors and ocular pathology.
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