加法器
流离失所(心理学)
DNA
分支迁移
电子线路
灵活性(工程)
生物系统
DNA纳米技术
纳米技术
功能(生物学)
计算机科学
生物物理学
化学
物理
材料科学
生物
数学
遗传学
量子力学
生物化学
电信
延迟(音频)
心理学
同源重组
统计
心理治疗师
霍利迪路口
作者
Shulian Bai,Bangtian Xu,Jiangling Wu,Guoming Xie
标识
DOI:10.1016/j.bios.2023.115677
摘要
Toehold-mediated strand displacement (TMSD) is widely employed in constructing a wide range of chemical reaction networks. In TMSD, single-stranded DNA or RNA can fold back upon itself to form a local short double-strand structure often hindering bimolecular hybridization. Here, based on series and parallel circuits, we introduce two mechanisms: series toehold-mediated strand displacement (STMSD) and parallel toehold-mediated strand displacement (PTMSD). These mechanisms can be highly effective when the target area is blocked by a secondary structure. In addition, these systems allow regulating the reaction rates spanning three to five orders of magnitude by adjusting the length of the two toeholds with the added advantage of multifunctional regulation and selectivity. To demonstrate the impressive function of this approach, a logic operation system based on STMSD was constructed to simulate the signal processing of a half-adder. We believe that the introduction of series and parallel toeholds will provide design flexibility contributing to the development of molecular computers, molecular robotics, and DNA-based biosensors.
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