微型反应器
纳米技术
微流控
材料科学
壳聚糖
生物催化
酶催化
化学工程
固定化酶
催化作用
化学
酶
有机化学
反应机理
工程类
作者
Jiangjuan Yuan,Huan Kan,Han Wang,Li Wang,Yun Liu,Dong Pei,Qingli Qu
标识
DOI:10.1016/j.coco.2023.101765
摘要
Enzymatic microreactors have gradually become a research hotspot as they can provide an ideal environment for the enzymatic reaction to ensure that the enzymatic reaction can be carried out in an organized manner. Here, inspired by the cellular structure of eukaryotic organisms, we developed Alginate@Chitosan (Alg@CS) microparticles for enzymatic reactions by oil-free gas-shearing method, allowing free access to substrate and product while shielding the enzyme from its surroundings. With the interpenetrating network crosslinked by Alg, CS and Ca2+ as the substrate, XOD can be encapsulated in particles at the same time of crosslinking. The microreactors are more stable and could provide more active sites for XOD with the addition of CS. The microreactors show excellent recyclability and 4 °C storage stability. This work opens up an intriguing avenue for enzyme microreactor preparation, which is important for the construction of artificial biomimetic catalytic systems.
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