电化学发光
发光体
化学
检出限
组合化学
过氧二硫酸盐
免疫分析
纳米技术
催化作用
材料科学
色谱法
发光
有机化学
抗体
免疫学
光电子学
生物
作者
Yuxuan Dai,Yixuan Li,Jérôme Chauvin,Xueji Zhang,Serge Cosnier,Robert S. Marks,Dan Shan
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2024-03-27
标识
DOI:10.1021/acssensors.4c00359
摘要
Efficient and robust electrochemiluminescence (ECL) emitters are crucial for enhancing the ECL immunosensor sensitivity. This study introduces a novel ECL emitter, CoBIM/Cys, featuring a hierarchical core-shell structure. The core of the structure is created through the swift coordination between the sulfhydryl and carboxyl groups of l-cysteine (l-Cys) and cobalt ions (Co2+), while the shell is constructed by sequentially coordinating benzimidazole (BIM) with Co2+. This design yields a greater specific surface area and a more intricate porous structure compared to CoBIM, markedly enhancing mass transfer and luminophore accessibility. Moreover, the l-Cys and Co2+ core introduces Co-S and Co-O catalytic sites, which improve the catalytic decomposition of H2O2, leading to an increased production of hydroperoxyl radicals (OOH•). This mechanism substantially amplifies the ECL performance. Leveraging the competitive interaction between isoluminol and BIM for OOH• during ECL emission, we developed a ratiometric immunosensor for cardiac troponin I (cTnI) detection. This immunosensor demonstrates a remarkably broad detection range (1 pg mL-1 to 10 ng mL-1), a low detection limit (0.4 pg mL-1), and exceptional reproducibility and specificity.
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