RNA沉默
RNA干扰
核糖核酸
基因沉默
生物
细胞生物学
化学
小干扰RNA
生物化学
基因
作者
Neena Mitter,Elizabeth A. Worrall,Karl E. Robinson,Peng Li,Ritesh G. Jain,Christelle Taochy,Stephen J. Fletcher,Bernard J. Carroll,Gao Qing Lu,Zhi Ping Xu
出处
期刊:Nature plants
[Springer Nature]
日期:2017-01-09
卷期号:3 (2)
被引量:744
标识
DOI:10.1038/nplants.2016.207
摘要
Topical application of pathogen-specific double-stranded RNA (dsRNA) for virus resistance in plants represents an attractive alternative to transgenic RNA interference (RNAi). However, the instability of naked dsRNA sprayed on plants has been a major challenge towards its practical application. We demonstrate that dsRNA can be loaded on designer, non-toxic, degradable, layered double hydroxide (LDH) clay nanosheets. Once loaded on LDH, the dsRNA does not wash off, shows sustained release and can be detected on sprayed leaves even 30 days after application. We provide evidence for the degradation of LDH, dsRNA uptake in plant cells and silencing of homologous RNA on topical application. Significantly, a single spray of dsRNA loaded on LDH (BioClay) afforded virus protection for at least 20 days when challenged on sprayed and newly emerged unsprayed leaves. This innovation translates nanotechnology developed for delivery of RNAi for human therapeutics to use in crop protection as an environmentally sustainable and easy to adopt topical spray.
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