Molecular characterization of African Swine fever viruses in Burkina Faso, Mali, and Senegal 1989–2016

Transboundary and Emerging DiseasesVolume 68, Issue 5 p. 2842-2852 SPECIAL ISSUE ARTICLE Molecular characterization of African Swine fever viruses in Burkina Faso, Mali, and Senegal 1989–2016 Genetic diversity of ASFV in West Africa Germaine L. Minoungou, Germaine L. Minoungou Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina FasoSearch for more papers by this authorMariame Diop, Mariame Diop Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, SénégalSearch for more papers by this authorMarthin Dakouo, Marthin Dakouo Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorAbdoul Karim Ouattara, Corresponding Author Abdoul Karim Ouattara [email protected] orcid.org/0000-0002-9767-0646 Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Biomolecular Research Center Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso Correspondence Dr Abdoul Karim Ouattara, Ph.D. in Genetics and Molecular Biology. CERBA/LABIOGENE, UFR/SVT, University Joseph Ki-Zerbo, 01 BP 364, Ouagadougou 01, Burkina Faso. Email: [email protected]Search for more papers by this authorTirumala Bharani K. Settypalli, Tirumala Bharani K. Settypalli Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, AustriaSearch for more papers by this authorModou M. Lo, Modou M. Lo Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, SénégalSearch for more papers by this authorSatigui Sidibe, Satigui Sidibe Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorEstelle Kanyala, Estelle Kanyala Direction de la Santé Animale (DSA), Ouagadougou, Burkina FasoSearch for more papers by this authorYaya Sidi Kone, Yaya Sidi Kone Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorMoctar Sidi Diallo, Moctar Sidi Diallo Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina Faso DeceasedSearch for more papers by this authorAnne Ouedraogo, Anne Ouedraogo Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina FasoSearch for more papers by this authorKadiatou Coulibaly, Kadiatou Coulibaly Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorVictorine Ouedraogo, Victorine Ouedraogo Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina FasoSearch for more papers by this authorIbrahim Sow, Ibrahim Sow Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorMamadou Niang, Mamadou Niang Laboratoire Central Vétérinaire (LCV), Bamako, Mali Emergency Center for Transboundary Animal Diseases (ECTAD), Regional Office for Africa (RAF), Food and Agriculture Organization of the United Nations (FAO), Accra, GhanaSearch for more papers by this authorJenna Elizabeth Achenbach, Jenna Elizabeth Achenbach Battelle Memorial Institute, Charlottesville, Virgina, USASearch for more papers by this authorAbel Wade, Abel Wade National Veterinary Laboratory (LANAVET), Garoua, CameroonSearch for more papers by this authorHermann Unger, Hermann Unger Animal Production and Health Section, Joint FAO/IAEA Division for Nuclear Applications in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International Centre, Vienna, AustriaSearch for more papers by this authorAdama Diallo, Adama Diallo Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, Sénégal Cirad, UMR CIRAD INRA, Animal, Santé, Territoires, Risques et Ecosystèmes (ASTRE), Campus International de Baillarguet, Montpellier, FranceSearch for more papers by this authorGiovanni Cattoli, Giovanni Cattoli Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, AustriaSearch for more papers by this authorCharles Euloge Lamien, Charles Euloge Lamien orcid.org/0000-0003-1899-7614 Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, AustriaSearch for more papers by this authorJacques Simpore, Jacques Simpore Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Biomolecular Research Center Pietro Annigoni (CERBA), Ouagadougou, Burkina FasoSearch for more papers by this author Germaine L. Minoungou, Germaine L. Minoungou Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina FasoSearch for more papers by this authorMariame Diop, Mariame Diop Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, SénégalSearch for more papers by this authorMarthin Dakouo, Marthin Dakouo Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorAbdoul Karim Ouattara, Corresponding Author Abdoul Karim Ouattara [email protected] orcid.org/0000-0002-9767-0646 Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Biomolecular Research Center Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso Correspondence Dr Abdoul Karim Ouattara, Ph.D. in Genetics and Molecular Biology. CERBA/LABIOGENE, UFR/SVT, University Joseph Ki-Zerbo, 01 BP 364, Ouagadougou 01, Burkina Faso. Email: [email protected]Search for more papers by this authorTirumala Bharani K. Settypalli, Tirumala Bharani K. Settypalli Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, AustriaSearch for more papers by this authorModou M. Lo, Modou M. Lo Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, SénégalSearch for more papers by this authorSatigui Sidibe, Satigui Sidibe Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorEstelle Kanyala, Estelle Kanyala Direction de la Santé Animale (DSA), Ouagadougou, Burkina FasoSearch for more papers by this authorYaya Sidi Kone, Yaya Sidi Kone Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorMoctar Sidi Diallo, Moctar Sidi Diallo Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina Faso DeceasedSearch for more papers by this authorAnne Ouedraogo, Anne Ouedraogo Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina FasoSearch for more papers by this authorKadiatou Coulibaly, Kadiatou Coulibaly Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorVictorine Ouedraogo, Victorine Ouedraogo Laboratoire National d'Elevage (LNE), Ouagadougou, Burkina FasoSearch for more papers by this authorIbrahim Sow, Ibrahim Sow Laboratoire Central Vétérinaire (LCV), Bamako, MaliSearch for more papers by this authorMamadou Niang, Mamadou Niang Laboratoire Central Vétérinaire (LCV), Bamako, Mali Emergency Center for Transboundary Animal Diseases (ECTAD), Regional Office for Africa (RAF), Food and Agriculture Organization of the United Nations (FAO), Accra, GhanaSearch for more papers by this authorJenna Elizabeth Achenbach, Jenna Elizabeth Achenbach Battelle Memorial Institute, Charlottesville, Virgina, USASearch for more papers by this authorAbel Wade, Abel Wade National Veterinary Laboratory (LANAVET), Garoua, CameroonSearch for more papers by this authorHermann Unger, Hermann Unger Animal Production and Health Section, Joint FAO/IAEA Division for Nuclear Applications in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International Centre, Vienna, AustriaSearch for more papers by this authorAdama Diallo, Adama Diallo Laboratoire National de l'Elevage et de Recherches Vétérinaires (LNERV), Institut Sénégalais de Recherches Agricoles (ISRA), Dakar, Sénégal Cirad, UMR CIRAD INRA, Animal, Santé, Territoires, Risques et Ecosystèmes (ASTRE), Campus International de Baillarguet, Montpellier, FranceSearch for more papers by this authorGiovanni Cattoli, Giovanni Cattoli Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, AustriaSearch for more papers by this authorCharles Euloge Lamien, Charles Euloge Lamien orcid.org/0000-0003-1899-7614 Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, AustriaSearch for more papers by this authorJacques Simpore, Jacques Simpore Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Biomolecular Research Center Pietro Annigoni (CERBA), Ouagadougou, Burkina FasoSearch for more papers by this author First published: 29 July 2021 https://doi.org/10.1111/tbed.14240Citations: 5 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary African swine fever (ASF) has been endemic in sub-Saharan Africa since the 1960s. Following its introduction in Senegal, in 1957, ASF steadily progressed through West Africa, reaching Burkina Faso in 2003, and later Mali in 2016. Despite the heavy burden of disease on pig production, little information is available on the genetic diversity of Africa swine fever virus (ASFV) in Burkina Faso, Mali and Senegal. Here, we used real-time PCR ASFV to detect the ASFV genome in samples collected between 1989 and 2016, in Burkina Faso, Mali and Senegal, and conventional approaches for isolate characterization. The C-terminal end of the p72 protein gene, the full E183L gene and the central variable region (CVR) within the B602L gene in ASFV genome were sequenced and compared to publicly available sequences. ASFV genome was found in 27 samples, 19 from Burkina Faso, three from Mali and five from Senegal. The phylogenetic analyses showed that all viruses belong to genotype I, with the ASFVs from Burkina Faso and Mali grouping with genotype Ia and ASFV serogroup 4, and those from Senegal with genotype Ib and the ASFV serogroup 1. The analysis of the CVR tetrameric tandem repeat sequences (TRS) showed four TRS variants in Burkina Faso, two in Senegal and one in Mali. The three countries did not share any common TRS, and all CVRs of this study differed from previously reported CVRs in West Africa, except for Senegal. Three of the five isolates from Senegal fully matched with the CVR, p72 and p54 sequences from ASFV IC96 collected during the 1996 ASF outbreak in Ivory Coast. This study shows the spread of the same ASFV strains across countries, highlighting the importance of continuous monitoring of ASFV isolates. It also calls for an urgent need to establish a regional plan for the control and eradication of ASF in West Africa. CONFLICT OF INTEREST The authors declare that there is no conflict of interest. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are openly available in NCBI at https://www.ncbi.nlm.nih.gov/nuccore/ Citing Literature Supporting Information Filename Description tbed14240-sup-0001-FigureS1.tif907.4 KB SUPPORTING INFORMATION tbed14240-sup-0002-TableS1.docx13.1 KB SUPPORTING INFORMATION Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume68, Issue5Special Issue: African Swine Fever – The Forgotten PandemicSeptember 2021Pages 2842-2852 RelatedInformation