Detection of Protein–Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique

Pull-down assay is a technique to analyze direct protein–protein interaction under in vitro condition. Also, this technique is appropriate for investigating the direct interaction between two purified proteins. Glutathione-s-transferase (GST) protein is a widely used affinity tag for affinity purification. In this chapter, we explain the widely used GST pull-down assay to identify the protein–protein interaction between purified proteins.