First Report of Capsicum chlorosis orthotospovirus Infecting Zucchini (Cucurbita pepo) in China

HomePlant DiseaseVol. 102, No. 10First Report of Capsicum chlorosis orthotospovirus Infecting Zucchini (Cucurbita pepo) in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Capsicum chlorosis orthotospovirus Infecting Zucchini (Cucurbita pepo) in ChinaS. E. Sun, J. Q. Wang, S. Chen, S. B. Zhang, D. Y. Zhang, and Y. LiuS. E. SunSearch for more papers by this author, J. Q. WangSearch for more papers by this author, S. ChenSearch for more papers by this author, S. B. ZhangSearch for more papers by this author, D. Y. Zhang†Corresponding authors: D. Y. Zhang; E-mail: E-mail Address: dyzhang78@163.com and Y. Liu; E-mail: E-mail Address: haoasliu@163.comhttp://orcid.org/0000-0002-5017-1911Search for more papers by this author, and Y. Liu†Corresponding authors: D. Y. Zhang; E-mail: E-mail Address: dyzhang78@163.com and Y. Liu; E-mail: E-mail Address: haoasliu@163.comSearch for more papers by this authorAffiliationsAuthors and Affiliations S. E. Sun , Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China J. Q. Wang , Hunan Agricultural University, Changsha 410128, China S. Chen S. B. Zhang D. Y. Zhang † Y. Liu † , Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China. Published Online:14 Aug 2018https://doi.org/10.1094/PDIS-12-17-1876-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Capsicum chlorosis orthotospovirus (CaCV), mostly transmitted by Frankliniella occidentalis (Thripidae, Frankliniella), a tentative species in the genus Orthotospovirus, family Tospoviridae, was first identified in tomato in Australia in 2002 (McMichael et al. 2002). To date, CaCV has been reported to infect peanut (Chen et al. 2007) and tomato (Huang et al. 2010; Yin et al. 2016) in China. During a survey of zucchini (Cucurbita pepo L.) viral diseases in October 2015, about 30% of fruits had chlorosis and rugged symptoms in Shouguang City, Shandong Province, China. The symptomatic fruits of zucchini were collected and tested for virus identification using high-throughput small RNA sequencing. Small unique RNA reads (17 to 28 nt) were assembled into contigs using Velvet 1.0.5 software. BLASTn searches of the resultant contigs in the National Center for Biotechnology Information databases identified about 40 contigs matching four viruses (watermelon mosaic virus, capsicum chlorosis orthotospovirus, watermelon silver mottle virus, and peanut bud necrosis virus) with 86 to 100, 100, 96, and 83% sequence identity, respectively. Among them, one contig nucleotide sequence (50 bp) had 100% identity with the corresponding regions of CaCV M segment genome (KX078566). To confirm this, reverse transcription polymerase chain reaction was conducted with a primer pair CaCV-F (5′-ACTTTCCATCAACCTCTGT-3′) and CaCV-R (5′-GTTATGGCCATATTTCCCT-3′) in eight symptomatic zucchini samples. An expected 812-bp fragment, partial sequence of GN/GC gene on the M RNA, was amplified in one-half of symptomatic zucchini samples and not in a healthy sample. The fragment was purified and sequenced, and the sequence was deposited in GenBank (accession no. MG244190). The nucleotide identity of the amplified fragment was 99% with CaCV-Hainan isolate from Arachnis labrosa (KX078566) and Qld-3432 isolate from pepper in Australia (KM589494), 98% with isolate from Taiwan (KC953854), and 97% with isolate from India (KX499515). The presence of CaCV in the eight symptomatic zucchini samples was verified by using ELISA with CaCV-specific polyclonal antibody (DSMZ, Germany). The infection of pepper (Krishnareddy et al. 2008), tomato (Premachandra et al. 2005), groundnut (Vijayalakshmi et al. 2016), and waxflower (Melzer et al. 2014) with CaCV was previously reported from Australia, Thailand, India, and the United States. No natural infection of zucchini or other cucurbit crops by CaCV has been reported. To our knowledge, this is the first report of CaCV infection of zucchini in China. This study suggests that CaCV may be more widely distributed in China than previously thought.References:Chen, K., et al. 2007. J. Phytopathol. 155:178. https://doi.org/10.1111/j.1439-0434.2007.01217.x Crossref, ISI, Google ScholarHuang, C. H., et al. 2010. Plant Dis. 94:1263. https://doi.org/10.1094/PDIS-04-10-0275 Link, ISI, Google ScholarKrishnareddy, M., et al. 2008. Plant Dis. 92:1469. https://doi.org/10.1094/PDIS-92-10-1469B Link, ISI, Google ScholarMcMichael, L. A., et al. 2002. Australas. Plant Pathol. 31:231. https://doi.org/10.1071/AP02016 Crossref, ISI, Google ScholarMelzer, M. J., et al. 2014. Plant Dis. 98:571. https://doi.org/10.1094/PDIS-06-13-0588-PDN Link, ISI, Google ScholarPremachandra, W. T. S. D., et al. 2005. Phytopathol. 95:659. https://doi.org/10.1094/PHYTO-95-0659 Link, ISI, Google ScholarVijayalakshmi, G., et al. 2016. Plant Dis. 100:2339. https://doi.org/10.1094/PDIS-05-16-0595-PDN Link, Google ScholarYin, Y. Y., et al. 2016. Plant Dis. 100:230. https://doi.org/10.1094/PDIS-01-15-0088-PDN Link, Google ScholarFunding: Funding was provided by Special Fund for Agro-scientific Research in the Public Interest (grant no. 201303028) and Hunan Provincial Natural Science Foundation of China (grant no. 2016JJ6067).S. E. Sun and J. Q. Wang contributed equally to this manuscript.DetailsFiguresLiterature CitedRelated Vol. 102, No. 10 October 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 28 Sep 2018Published: 14 Aug 2018First Look: 24 Apr 2018Accepted: 20 Apr 2018 Pages: 2049-2049 Information© 2018 The American Phytopathological SocietyFundingSpecial Fund for Agro-scientific Research in the Public InterestGrant/Award Number: 201303028Hunan Provincial Natural Science Foundation of ChinaGrant/Award Number: 2016JJ6067Cited byPest categorisation of Capsicum chlorosis virusEFSA Journal, Vol. 20, No. 6Small RNA deep sequencing reveals the presence of multiple viral infections in cucurbit crops in Guangdong, ChinaJournal of Integrative Agriculture, Vol. 21, No. 5Temporal expression of defence and susceptibility genes and tospovirus accumulation in capsicum chlorosis virus-infected capsicum4 March 2022 | Archives of Virology, Vol. 167, No. 4Capsicum chlorosis virusCABI Compendium, Vol. CABI CompendiumCucurbita pepo (Summer squash, Zucchini, Vegetable marrow)6 June 2020