乳酸乳球菌
免疫系统
dna疫苗
生物
抗原
增强剂量
病毒学
ESAT-6号
结核病疫苗
免疫学
免疫原性
微生物学
结核分枝杆菌
免疫
肺结核
重组DNA
医学
基因
细菌
遗传学
病理
乳酸
作者
Vanessa Bastos Pereira,Vanessa Pecini da Cunha,Tatiane Melo Preisser,Bianca Mendes Souza,Meritxell Zurita-Turk,Camila Prósperi de Castro,Marcela de Azevedo,Anderson Miyoshi
摘要
A regimen utilizing Bacille Calmette-Guerin (BCG) and another vaccine system as a booster may represent a promising strategy for the development of an efficient tuberculosis vaccine for adults. In a previous work, we confirmed the ability of Lactococcus lactis fibronectin-binding protein A (FnBPA+) (pValac:ESAT-6), a live mucosal DNA vaccine, to produce a specific immune response in mice after oral immunization. In this study, we examined the immunogenicity of this strain as a booster for the BCG vaccine in mice.After immunization, cytokine and immunoglobulin profiles were measured. The BCG prime L. lactis FnBPA+ (pValac:ESAT-6) boost group was the most responsive group, with a significant increase in splenic pro-inflammatory cytokines IL-17, IFN-γ, IL-6 and TNF-α compared with the negative control.Based on the results obtained here, we demonstrated that L. lactis FnBPA+ (pValac:ESAT-6) was able to increase the BCG vaccine general immune response.This work is of great scientific and social importance because it represents the first step towards the development of a booster to the BCG vaccine using L. lactis as a DNA delivery system.
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