生物传感器
校准
方波
化学
信号(编程语言)
电化学气体传感器
动态范围
准确度和精密度
分析化学(期刊)
生物系统
电极
电化学
计算机科学
光学
物理
色谱法
电压
物理化学
生物
量子力学
程序设计语言
生物化学
作者
Hui Li,Philippe Dauphin‐Ducharme,Gabriel Ortega,Kevin W. Plaxco
摘要
The need to calibrate to correct for sensor-to-sensor fabrication variation and sensor drift has proven a significant hurdle in the widespread use of biosensors. To maintain clinically relevant (±20% for this application) accuracy, for example, commercial continuous glucose monitors require recalibration several times a day, decreasing convenience and increasing the chance of user errors. Here, however, we demonstrate a "dual-frequency" approach for achieving the calibration-free operation of electrochemical biosensors that generate an output by using square-wave voltammetry to monitor binding-induced changes in electron transfer kinetics. Specifically, we use the square-wave frequency dependence of their response to produce a ratiometric signal, the ratio of peak currents collected at responsive and non- (or low) responsive square-wave frequencies, which is largely insensitive to drift and sensor-to-sensor fabrication variations. Using electrochemical aptamer-based (E-AB) biosensors as our test bed, we demonstrate the accurate and precise operation of sensors against multiple drugs, achieving accuracy in the measurement of their targets of within better than 20% across dynamic ranges of up to 2 orders of magnitude without the need to calibrate each individual sensor.
科研通智能强力驱动
Strongly Powered by AbleSci AI