细胞凋亡
活力测定
结直肠癌
MTT法
甲氨蝶呤
癌细胞
药物输送
药理学
化学
体外
癌症研究
材料科学
癌症
分子生物学
医学
纳米技术
生物化学
生物
免疫学
内科学
作者
Zahra Khatibi,Negar Motakef Kazemi,Sepideh Khaleghi
标识
DOI:10.1016/j.jddst.2022.103441
摘要
Colon cancer has the highest death rate all around the world and conventional therapeutic approaches are not already terminated the colon cancer prevalence. Thus, a novel folic acid (FA)-chitosan (CS)-coated nanoscale metal-organic frameworks (NMOFs) was developed in this study for active delivery of methotrexate (MTX) to colon cancer cells. After qualification and quantification of synthesized MOF-CS-FA-MTX nanocomposite via FT-IR, XRD, DLS, SEM, and TEM devices and verifying the nano-size diameter of the nanocomposite, drug loading capacity (78%) and drug release manner (10% ˃ within first 5h) were measured in vitro. MTX release was indicated pH sensitivity and its release was about 10 folds higher in acidic pH than normal pH within the first 5 h. The MTT assay was indicated less than 5% cell viability in HCT116 cells after treatment with a 2.5 ng/mL concentration of MOF-CS-FA-MTX after 48 h of treatment. The gene expression study was exhibited a 6-fold increase in BAX and a 2-fold decline in Bcl2 genes expression, and BECLIN1 and ATG5 autophagy genes were also shown a nearly 5-fold rise in their expression after treatment with 5 ng/mL MOF-CS-FA-MTX. The cell cycle arrest (15.49% apoptosis) and apoptosis (20% apoptosis) assays during 5 h were potentially approved for the cancer cell inhibition ability of MOF-CS-FA-MTX. These outputs have been presenting a remarkable potency of NMOF-CS-FA-MTX in suppressing colon cancer cells.
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