Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

AbstractAbstractThe aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), production of reactive oxygen species (ROS), and total antioxidant status (TAS) in porcine ovarian granulosa cells after quercetin and T-2 toxin exposure in vitro. Porcine ovarian granulosa cells were incubated with quercetin and T-2 toxin separately or in mutual combination at the doses of 1ng/mL, 10 ng/mL, 100 ng/mL, and the control group without any additions for 24 h. In this study T-2 toxin developed stress reaction in porcine ovarian granulosa cells and increased generation of ROS. Quercetin had no effect in elimination of ROS generation induced by T-2 toxin, but was effective in maintaining and increasing of TAS, activities of SOD and GPx in porcine granulosa cells in vitro. These results contribute towards the understanding of cellular stress and its response.Keywords: Porcine granulosa cellsquercetinT-2 toxinreactive oxygen speciesantioxidants FundingThis work was financially supported by the scientific grant agency of the Ministry of Education of the Slovak Republic (ME SR) and of Slovak Academy of Sciences (SAS) VEGA project no. 1/0084/12.