Colorimetric analyzer based on mobile phone camera for determination of available phosphorus in soil

化学 钼蓝 钼酸铵 频谱分析仪 磷钼酸 RGB颜色模型 准确度和精密度 抗坏血酸 吸光度 分析化学(期刊) 色谱法 计算机科学 人工智能 电信 生物化学 统计 数学 食品科学 有机化学 催化作用
作者
Nuntaporn Moonrungsee,Somkid Pencharee,Jaroon Jakmunee
出处
期刊:Talanta [Elsevier BV]
卷期号:136: 204-209 被引量:112
标识
DOI:10.1016/j.talanta.2015.01.024
摘要

A field deployable colorimetric analyzer based on an "Android mobile phone" was developed for the determination of available phosphorus content in soil. An inexpensive mobile phone embedded with digital camera was used for taking photograph of the chemical solution under test. The method involved a reaction of the phosphorus (orthophosphate form), ammonium molybdate and potassium antimonyl tartrate to form phosphomolybdic acid which was reduced by ascorbic acid to produce the intense colored molybdenum blue. The software program was developed to use with the phone for recording and analyzing RGB color of the picture. A light tight box with LED light to control illumination was fabricated to improve precision and accuracy of the measurement. Under the optimum conditions, the calibration graph was created by measuring blue color intensity of a series of standard phosphorus solution (0.0–1.0 mg P L−1), then, the calibration equation obtained was retained by the program for the analysis of sample solution. The results obtained from the proposed method agreed well with the spectrophotometric method, with a detection limit of 0.01 mg P L−1 and a sample throughput about 40 h−1 was achieved. The developed system provided good accuracy (RE<5%) and precision (RSD<2%, intra- and inter-day), fast and cheap analysis, and especially convenient to use in crop field for soil analysis of phosphorus nutrient.
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