Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix - based scaffold

牙囊 牙本质 牙骨质 釉质基质衍生物 成牙本质细胞 细胞外基质 材料科学 再生(生物学) 牙本质涎磷蛋白 细胞生物学 纤维连接蛋白 生物医学工程 组织工程 脚手架 牙髓干细胞 牙科 干细胞 生物 医学
作者
Bo Yang,Gang Chen,Jie Li,Qing Zou,Dan Xie,Yali Chen,Hang Wang,Xiaohui Zheng,Jie Long,Wei Tang,Weihua Guo,Weidong Tian
出处
期刊:Biomaterials [Elsevier]
卷期号:33 (8): 2449-2461 被引量:146
标识
DOI:10.1016/j.biomaterials.2011.11.074
摘要

Stem cell mediated tissue engineering has been acknowledged as a prospective strategy for repairing and replacing damaged and lost tissues. However, the low survival rate of implanted stem cells proves to be a major challenge in the management of transplantation failures. While previous studies have indicated the effectiveness of tissue engineered cell sheets in improving the survival rate of implanted cells, we have recently demonstrated the use of treated dentin matrix (TDM) as a biological scaffold and dental follicle cells (DFCs) as the seeding cells for dentinogenesis and tooth root construction. This study proposes a strategy utilizing TDM with human dental follicle cell sheets (DFCSs) for root regeneration. The biological characteristics and changes of human DFCSs under the effect of TDM were studied with scanning electron microscopy, transmission electron microscopy, immunofluorescence microscopy, immunohistochemistry and quantitative real-time PCR. DFCSs combined with TDM were implanted subcutaneously into the dorsum of mice. Histological examination of the harvested grafts revealed a whirlpool-like alignment of the DFCs in multiple layers that were positive for COLI, integrinβ1, fibronectin and alkaline phosphatase (ALP), suggestive of the formation of a rich extracellular matrix. DFCSs, under the effect of TDM, highly expressed DMP-1 and bone sialoprotein (BSP), indicating their potential for odontogenesis and osteogenesis. Importantly, in vivo, TDM could induce and support DFCSs to develop new dentin-pulp like tissues and cementum-periodontal complexes that were positive for markers such as DSP, nestin and VIII factors, COLI and cementum attachment protein (CAP), implying successful root regeneration. Therefore, DFCSs combined with TDM may prove to be a better strategy for the construction of tooth root, and is a prospective approach that could be utilized for the treatment of root or tooth defect or loss in future.
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