腐蚀性艾克奈拉
干酪乳杆菌
变形链球菌
微生物学
比色法
细菌
化学
显色的
硫化氢
唾液
色谱法
食品科学
生物
生物化学
有机化学
硫黄
发酵
遗传学
作者
Byung-Ki Ahn,Yong-Jin Ahn,Young-Ju Lee,Yeon‐Hee Lee,Gi‐Ja Lee
出处
期刊:Sensors
[MDPI AG]
日期:2022-08-08
卷期号:22 (15): 5928-5928
被引量:5
摘要
Hydrogen sulfide (H2S) is known to participate in bacteria-induced inflammatory response in periodontal diseases. Therefore, it is necessary to quantify H2S produced by oral bacteria for diagnosis and treatment of oral diseases including halitosis and periodontal disease. In this study, we introduce a paper-based colorimetric assay for detecting bacterial H2S utilizing silver/Nafion/polyvinylpyrrolidone membrane and a 96-well microplate. This H2S-sensing paper showed a good sensitivity (8.27 blue channel intensity/μM H2S, R2 = 0.9996), which was higher than that of lead acetate paper (6.05 blue channel intensity/μM H2S, R2 = 0.9959). We analyzed the difference in H2S concentration released from four kinds of oral bacteria (Eikenella corrodens, Streptococcus sobrinus, Streptococcus mutans, and Lactobacillus casei). Finally, the H2S level in Eikenella corrodens while varying the concentration of cysteine and treatment time was quantified. This paper-based colorimetric assay can be utilized as a simple and effective tool for in vitro screening of H2S-producing ability of many bacteria as well as salivary H2S analysis.
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