亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Cellulose Nanocrystal-Immobilized Lipase for Pickering Interface Biocatalysis

生物催化 纳米晶 纤维素 脂肪酶 皮克林乳液 纳米技术 仿生学 化学 材料科学 化学工程 催化作用 有机化学 纳米颗粒 离子液体 工程类
作者
Lili Li,Xiaojing Wang,Yali Hu,Wang Sun,Yugao Ding,Nisha He,Guofu Zhou,Zhen Zhang
出处
期刊:ACS Sustainable Chemistry & Engineering [American Chemical Society]
被引量:7
标识
DOI:10.1021/acssuschemeng.4c07051
摘要

Enzymes are promising biocatalysts due to their high efficiency, mild conditions, high specificity, nontoxicity, and environmental friendliness. However, the enzyme suffers from poor stability and reusability. Enzyme immobilization is a commonly used technology to enhance its stability and reusability. In this study, the cellulose nanocrystal (CNC) was chosen as the enzyme immobilization carrier due to its sustainability, high specific surface area, biocompatibility, water dispersibility, and excellent Pickering emulsifying ability. Lipase was immobilized on a polydopamine-coated CNC (PC), and the obtained lipase-immobilized PC (LPC) displayed significantly enhanced stability and higher activity in harsh conditions compared to free lipase. LPC possessed partial wettability with both water and oil phases and a lower absolute value of the zeta potential than CNC, endowing it with a better Pickering emulsifying ability than CNC. LPC was fixed at the oil–water interface of the Pickering emulsion by ultrasonication, and the resulting Pickering emulsion droplets were employed as microreactors for ester hydrolysis and synthesis via Pickering interface biocatalysis. LPC displayed significantly enhanced catalysis activity at the oil–water interface of the Pickering emulsion compared to free lipase in a traditional oil–water biphasic system due to the increased contact area between the enzyme and the substrate, reduced diffusion distance of the substrate, and lipase interface activation effect. Moreover, LPC could be facilely recycled by centrifugation, and the recycled LPC still retains high catalysis activity. This study proposed a facile and sustainable method of enzyme immobilization to enhance its stability and reusability and achieved a significantly increased activity via Pickering interface biocatalysis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小江完成签到,获得积分10
3秒前
gszy1975完成签到,获得积分10
7秒前
26秒前
28秒前
Chaos完成签到 ,获得积分10
33秒前
光合作用完成签到,获得积分10
36秒前
41秒前
55秒前
1分钟前
八个猪宝贝完成签到,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
lameliu完成签到,获得积分10
1分钟前
1分钟前
香蕉觅云应助Viiigo采纳,获得10
2分钟前
2分钟前
量子星尘发布了新的文献求助10
2分钟前
2分钟前
老石完成签到 ,获得积分10
3分钟前
chichqq发布了新的文献求助30
3分钟前
3分钟前
evil发布了新的文献求助10
3分钟前
3分钟前
珂珂完成签到 ,获得积分10
3分钟前
小蘑菇应助evil采纳,获得10
3分钟前
4分钟前
量子星尘发布了新的文献求助10
4分钟前
5分钟前
5分钟前
Dandelion完成签到,获得积分10
5分钟前
5分钟前
完美世界应助nsc采纳,获得10
5分钟前
量子星尘发布了新的文献求助10
5分钟前
所所应助nsc采纳,获得10
5分钟前
小蘑菇应助nsc采纳,获得50
5分钟前
Ava应助nsc采纳,获得10
5分钟前
隐形曼青应助nsc采纳,获得10
5分钟前
慕青应助nsc采纳,获得10
5分钟前
脑洞疼应助nsc采纳,获得10
5分钟前
善学以致用应助nsc采纳,获得10
5分钟前
ding应助nsc采纳,获得30
5分钟前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
T/CIET 1202-2025 可吸收再生氧化纤维素止血材料 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3957061
求助须知:如何正确求助?哪些是违规求助? 3503084
关于积分的说明 11111255
捐赠科研通 3234121
什么是DOI,文献DOI怎么找? 1787751
邀请新用户注册赠送积分活动 870762
科研通“疑难数据库(出版商)”最低求助积分说明 802264