电流体力学
微透镜
微流控
制作
纳米技术
喷射(流体)
材料科学
原位
3D打印
工程类
光学
化学
物理
复合材料
航空航天工程
镜头(地质)
电极
医学
替代医学
物理化学
病理
有机化学
作者
Ya Zhong,Haibo Yu,Peilin Zhou,Guo Hong-ji,Tianming Zhao,Yangdong Wen,Wuhao Zou,Lianqing Liu
摘要
Microfluidic systems have gained widespread attention in the biomedical field because of their ability to be integrated with microlenses, which can enhance cellular analysis and observation capabilities. However, the integration and design of microlenses in microfluidic systems for cell counting remain challenging. In this study, we developed a bioinspired microfluidic system with in situ-integrated microlens arrays (MLAs) to enable parallel, multichannel cell counting. Using microlenses enables non-contact cell counting by detecting light-intensity changes. Our self-developed, high-precision electrohydrodynamic jet (E-jet) printing system can achieve in situ microlens integration. The microfluidic system included nine channels, each with a microlens of 50 μm diameter printed on its central axis. Simulating and optimizing the microfluidic channel size enabled cells to align on the centerline of the channel and pass over the focal region of the microlens via inertial forces. As the cells flowed through the microlens, the intensity of the focal spots decreased by approximately 50%. Using our system, the success rate of cell counting in the microfluidic system reached 88.6%. The microfluidic cell-counting system can function independently or be integrated with other microfluidic systems as a unit, thus enhancing the single-cell operational and analytical capabilities of microfluidic systems.
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