化学
脱氧核酶
血红素
G-四倍体
原位
小RNA
电化学
纳米技术
纳米线
组合化学
色谱法
生物化学
电极
检出限
酶
DNA
有机化学
血红素
材料科学
物理化学
基因
作者
Tengteng Shan,Lingrong Cui,Huimin Zhang,Kaiqin Li,Jian‐Mei Yang,Yan Zhao,Yun Xiang,Ruo Yuan
出处
期刊:Talanta
[Elsevier]
日期:2024-09-19
卷期号:281: 126923-126923
标识
DOI:10.1016/j.talanta.2024.126923
摘要
The concentration elevation of myocardial microRNA (miRNA) biomarker is associated with the pathogenic process of acute myocardial infarction (AMI), and sensitive quantification of myocardial miRNA biomarker plays an important role for early AMI diagnosis and its treatment. In response, this work describes an ultrasensitive and non-label electrochemical biosensor for the assay of myocardial miRNA based on cascade signal amplifications integrated by DNAzyme walker and hemin/G-quadruplex nanowires. The DNAzyme walker is activated by presence of target miRNAs to move along the electrode surface to cyclically cleave the substrate hairpins to release G-quadruplex segments, which further trigger the in situ formation of many hemin/G-quadruplex nanowires. The large amounts of hemin intercalated into the DNA nanowires subsequently generate drastically magnified electrochemical current signals for highly sensitive label-free assay of myocardial miRNAs down to 15.7 fM within dynamic range of 100 fM to 10 nM. Such a biosensor also has high selectivity and can monitor myocardial miRNAs in diluted serums at low levels, providing a sensitive and reliable platform for diagnosing infarct-associated cardiovascular diseases.
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