京尼平
化学
明胶
骨钙素
骨桥蛋白
碱性磷酸酶
骨愈合
骨重建
生物化学
解剖
医学
内科学
酶
壳聚糖
作者
Yuan-Tsung Fu,Shi‐Yuan Sheu,Yueh-Sheng Chen,Kuo‐Yu Chen,Chun‐Hsu Yao
出处
期刊:Bone
[Elsevier]
日期:2015-09-01
卷期号:78: 15-22
被引量:23
标识
DOI:10.1016/j.bone.2015.04.034
摘要
Bone cell activities are very important in bone remodeling. This study investigates the effects of lumbrokinase on bone cell activities in cultures. Moreover, a biodegradable composite (GGT) containing genipin-crosslinked gelatin and β-tricalcium phosphate was prepared to carry lumbrokinase (GGTLK). Rat calvarial bone defects were filled with GGT and GGTLK composites. Bone healing was monitored in vivo by bioluminescence imaging and micro-CT. Lumbrokinase was found to have a dose-dependent effect on bone cell activities. Low concentrations (< 1 μg/ml) of lumbrokinase increased the viability, total alkaline phosphatase activity and mobility of osteoblasts, the number of total calcified nodules and the expression of osteopontin and osteocalcin; however, they considerably reduced the total tartrate-resistant acid phosphatase activity of osteoclasts. IVIS images revealed a stronger fluorescent signal in GGTLK-treated animals than in GGT-treated animals. Micro-CT analysis revealed that GGTLK induced more new bone formation than did GGT. These observations suggest that lumbrokinase released from GGTLK composite can enhance bone tissue regeneration.
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