脚手架
间充质干细胞
聚己内酯
材料科学
明胶
纳米纤维
组织工程
静电纺丝
生物医学工程
细胞生物学
生物物理学
纳米技术
化学
生物化学
复合材料
生物
聚合物
医学
作者
Shima Majidansari,Negin Vahedi,Mahmood Rekabgardan,Camellia Ganjoury,Najmeh Najmoddin,Mohammad Tabatabaei,Faraz Sigaroodi,Paniz Naraghi‐Bagherpour,Seyed Amir Ali Taheri,Mohammad‐Mehdi Khani
摘要
Abstract Cardiovascular diseases have always been one of the main causes of death worldwide and eventually one of the major medical concerns. Tissue engineering is promising strategies of treating cardiovascular, which can be an effective approach with the design of appropriate scaffold. In this study, to develop engineering basement membrane for endothelial differentiation with good cell attachment , we produced polycaprolactone (PCL)/poly (glycerol sebacate) (PGS)/gelatin nanofibrous scaffold via electrospinning. Attenuated total reflectance‐Fourier transform infrared and the proton nuclear magnetic resonance results confirmed the chemical structure of synthesized PGS. Scanning electron microscope images of the electrospun scaffold revealed that the nanofibers are smooth, continues and uniform. Moreover, due to the presence of hydrophilic functional groups in the scaffold, the contact angle is in the appropriate range for cell adhesion especially endothelial cells. The elastic modulus and ultimate tensile stress of electrospun scaffold were calculated 1.32 ± 0.27 MPa and 1.23 ± 0.18 MPa respectively. Quantitative polymerase chain reaction was performed for evaluation of endothelial differentiation of mesenchymal stem cells cultured on standard plate and fibrous scaffold under chemical stimulation with growth factor. Specific endothelial gene expression results postulated that our modified scaffold could support and significantly promote endothelial differentiation of MSCs.
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