犬尿氨酸
喹啉酸
化学
巴比妥酸
脑脊液
犬尿氨酸途径
单胺类神经递质
新喋呤
黄脲酸
邻氨基苯甲酸
色谱法
代谢物
衍生化
色氨酸
吡啶甲酸
血清素
生物化学
质谱法
氨基酸
医学
内科学
受体
作者
René Fuertig,Angelo Ceci,Sandrine Camus,Erwan Bézard,Andreas H. Luippold,Bastian Hengerer
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2016-09-01
卷期号:8 (18): 1903-1917
被引量:116
标识
DOI:10.4155/bio-2016-0111
摘要
The kynurenine (KYN) pathway is implicated in diseases such as cancer, psychiatric, neurodegenerative and autoimmune disorders. Measurement of KYN metabolite levels will help elucidating the involvement of the KYN pathway in the disease pathology and inform drug development.Samples of plasma, cerebrospinal fluid or brain tissue were spiked with deuterated internal standards, processed and analyzed by LC-MS/MS; analytes were chromatographically separated by gradient elution on a C18 reversed phase analytical column without derivatization.We established an LC-MS/MS method to measure 11 molecules, namely tryptophan, KYN, 3-OH-KYN, 3-OH-anthranilic acid, quinolinic acid, picolinic acid, kynurenic acid, xanthurenic acid, serotonin, dopamine and neopterin within 5.5 min, with sufficient sensitivity to quantify these molecules in small sample volumes of plasma, cerebrospinal fluid and brain tissue.
科研通智能强力驱动
Strongly Powered by AbleSci AI