膜
化学
牛血清白蛋白
色谱法
蛋白质吸附
化学工程
聚乙二醇
PEG比率
膜污染
结垢
膜结构
吸附
生物化学
有机化学
财务
工程类
经济
作者
Ningning Gao,Wenbin Xie,Lijing Xu,Qingping Xin,Junjun Gao,Jinjie Shi,Lin Zhong,Wenxiong Shi,Huiguo Wang,Kongyin Zhao,Ligang Lin
标识
DOI:10.1016/j.ijbiomac.2023.126367
摘要
The development of membranes for protein purification has stringent requirement of disinfection resistance, low protein adsorption and anti-fouling, without changing protein structure. In this study, hydrophilic titanium dioxide (TiO2)/calcium alginate (TiO2/CaAlg) hydrogel membranes were prepared by a simple ionic cross-linking method. The effects of the porogenic agent polyethylene glycol (PEG) concentration, the molecular weight of PEG, and the concentration of TiO2 on the filtration properties were systematically investigated. The TiO2/CaAlg membrane exhibited excellent bovine serum albumin (BSA) rejection and anti-fouling properties. The mechanical properties and surface energy of the TiO2/CaAlg membrane were significantly improved. The chemical bonding mechanism of TiO2 and NaAlg was investigated by molecular dynamic simulation. The TiO2/CaAlg membrane had good chlorine resistance and could be disinfected or cleaned with sodium hypochlorite. The TiO2/CaAlg hydrogel membrane loaded with polyhydroxybutyrate (PHB) nanofibers maintained high flux (136.7 L/m2h) and high BSA rejection (98.0 %) at 0.1 MPa. The results of circular dichroism and synchronous fluorescence indicated that the secondary structure of BSA was maintained after membrane separation. This study provides one method for the preparation of green and environmentally friendly membrane for protein purification.
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