芳香
梨
生物
水杨酸
转化(遗传学)
风味
龙葵
成熟
基因
异源表达
茉莉酸甲酯
食品科学
生物化学
植物
重组DNA
作者
Yangyang Chen,Xiao Wu,Xiaohua Wang,Yubo Yuan,Kaijie Qi,Shaoling Zhang,Hong Yin
标识
DOI:10.1016/j.jplph.2023.154101
摘要
Aroma is an important commercial trait that determines fruit quality and has an important influence on the overall flavor of fruits. Plant ALDH genes have been implicated in diverse pathways and play crucial roles in physiological activities. In this study, via genome resequencing we identified one gene PusALDH1 (Pbr034873.1) related to aroma biosynthesis that can respond to the induction of methyl jasmonate. Transient transformation of pear fruits and heterologous stable transformation of tomato further confirmed the function of PusALDH1 in aroma accumulation. The content of ALDH precursor substance, benzaldehyde, was reduced in the overexpressing pear and tomato fruits, and the content of ALDH product, benzoic acid and benzoic acid derivatives, was increased in the pear fruits. Meanwhile, transgenic tomato fruits with PusALDH1 overexpression exhibited a greater area of yellow placenta, indicating that the gene may be related to the growth and development of the fruit. Taken together, PusALDH1 could act as a strong candidate gene in aroma synthesis.
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