扫描离子电导显微镜
显微镜
电导
材料科学
扫描探针显微镜
离子
扫描共焦电子显微镜
光学
纳米技术
化学
物理
凝聚态物理
有机化学
作者
Shengbo Gu,Jian Zhuang,Tianying Wang,Shiting Hu,Wenwen Song,Xiaobo Liao
标识
DOI:10.1016/j.ultramic.2023.113910
摘要
Scanning ion conductance microscopy (SICM) has developed rapidly and has wide applications in biomedicine, single-cell science and other fields. SICM scanning speed is limited by the conventional raster-type scanning method, which spends most of time on imaging the substrate and does not focus enough on the target area. In order to solve this problem, a target region focused (TRF) method is proposed, which can effectively avoid the scanning of unnecessary substrate areas and enables SICM to image the target area only to achieve high-speed and effective local scanning. TRF method and conventional hopping mode scanning method are compared in the experiments using breast cancer cells and rat basophilic leukemia cells as experimental materials. It was demonstrated that our method can reduce the scanning time for a single sample image significantly without losing scanning information or compromising the quality of imaging. The TRF method developed in this paper can provide an efficient and fast scanning strategy for improving the imaging performance of SICM systems, which can be applied to the dynamic features of cell samples in the fields of biology and pharmacology analysis.
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