头颈部鳞状细胞癌
转移
癌相关成纤维细胞
癌症研究
细胞
基底细胞
头颈部
医学
癌症
头颈部癌
癌
病理
肿瘤科
生物
内科学
遗传学
外科
作者
Yihuan Yao,Guoli Tian,Jiaqiang Zhang,Shuaiyuan Zhang,Xiaoyong Liu,Jianxia Hou
出处
期刊:Life Sciences
[Elsevier]
日期:2024-08-01
卷期号:351: 122768-122768
标识
DOI:10.1016/j.lfs.2024.122768
摘要
Cancer-associated fibroblasts (CAFs) have been shown to promote the metastasis of head and neck squamous cell carcinoma (HNSCC), but the underlying mechanisms remain unclear. The purpose of this study is to identify gene in CAFs that are associated with metastasis and to preliminarily validate its impact on the metastasis of HNSCC. Scissor analysis was utilized to process single-cell and bulk RNA sequencing datasets, identifying genes associated with the metastasis of HNSCC through differential gene expression analysis. A risk model was constructed using LASSO regression analysis. Quantitative real time-PCR and Western blot were employed to measure mRNA and protein expressions, respectively. Multiplex immunohistochemistry (mIHC) was used to assess protein expression in CAFs. siRNA was utilized to achieve gene knockdown. CCK-8 and Transwell assays were employed to evaluate the biological characteristics of HNSCC cells. Compare to the nonmetastatic primary CAFs (nmCAFs), tissue inhibitors of metalloproteinase-1 (TIMP1) was founded to be overexpressed in the cells and tissues of metastatic primary CAFs (mCAFs). Knocking down TIMP1 in CAFs can signifucantly inhibit the proliferation, invasion, and migration of HNSCC cells. CAFs facilitate HNSCC cell metastasis by upregulating TIMP1, highlighting TIMP1 as a potential therapeutic target in HNSCC metastasis management.
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