生物
胚胎干细胞
造血
胚胎发生
中胚层
体细胞
遗传学
干细胞
胚胎
基因
作者
Michael Spencer Chapman,Anna Maria Ranzoni,Brynelle Myers,Nicholas Williams,Tim Coorens,Emily Mitchell,Timothy Butler,Kevin J. Dawson,Yvette Hooks,Luiza Moore,Jyoti Nangalia,Philip S. Robinson,Kenichi Yoshida,Elizabeth Hook,Peter J. Campbell,Ana Cvejic
出处
期刊:Nature
[Springer Nature]
日期:2021-05-12
卷期号:595 (7865): 85-90
被引量:99
标识
DOI:10.1038/s41586-021-03548-6
摘要
The ontogeny of the human haematopoietic system during fetal development has previously been characterized mainly through careful microscopic observations1. Here we reconstruct a phylogenetic tree of blood development using whole-genome sequencing of 511 single-cell-derived haematopoietic colonies from healthy human fetuses at 8 and 18 weeks after conception, coupled with deep targeted sequencing of tissues of known embryonic origin. We found that, in healthy fetuses, individual haematopoietic progenitors acquire tens of somatic mutations by 18 weeks after conception. We used these mutations as barcodes and timed the divergence of embryonic and extra-embryonic tissues during development, and estimated the number of blood antecedents at different stages of embryonic development. Our data support a hypoblast origin of the extra-embryonic mesoderm and primitive blood in humans. Whole-genome sequencing of haematopoietic colonies from human fetuses reveals the somatic mutations acquired by individual progenitors, which are used as barcodes to construct a phylogenetic tree of blood development.
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