Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

Strain TUM18999 T was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20–42 °C (optimum, 30–35 °C) in 1.0–4.0% NaCl (w/v) and at pH 5.5–9.5, optimum pH 5.5–8.5. The phylogenetic tree reconstructed using 16S rRNA, gyrB , rpoB and rpoD gene sequences indicated that strain TUM18999 T is closely related to Pseudomonas otitidis MCC10330 T . Although the partial 16S rRNA gene sequence (1412 bp) of TUM18999 T exhibits high similarity to those of Pseudomonas alcaligenes NBRC 14159 T (99.08 %) and Pseudomonas otitidis MCC10330 T (98.51 %), multi-locus sequence analysis using 16S rRNA, gyrB , rpoB and rpoD genes reveals a clear distinction between TUM18999 T and other Pseudomonas species. In addition, an average nucleotide identity >90 % was not observed in the P. aeruginosa group. Moreover, TUM18999 T and P. otitidis can be distinguished based on the minimum inhibitory concentration for carbapenem. Meanwhile, the cellular fatty acids are enriched with C 18 : 1 ω 7 c /C 18 : 1 ω 6 c (34.35 %), C 16 : 1 ω 7 c /C 16 : 1 ω 6 c (24.22 %), C 16 : 0 (19.79 %) and C 12 : 0 (8.25 %). Based on this evidence, strain TUM18999 T can be defined as representing a novel Pseudomonas species, with the proposed name Pseudomonas tohonis sp. nov. The type strain is TUM18999 T (GTC 22698 T =NCTC 14580 T ).