生物
底漆(化妆品)
真菌
植物
微生物学
园艺
有机化学
化学
作者
Chaoqun Tong,Tian Li,Shisui Luo,Ruoni Chen,Shijiang Chen,JunHong Wei,Yu-ling Qing,Shaorong Qin,Guoqing Pan,Chunfeng Li,Zeyang Zhou
出处
期刊:Canadian Journal of Microbiology
[Canadian Science Publishing]
日期:2023-01-13
卷期号:69 (3): 136-145
标识
DOI:10.1139/cjm-2022-0165
摘要
Cordyceps farinosa, an entomopathogenic fungus, infects and leads to high mortality of Thitarodes armoricanus larvae, which die soon after the infection of C. farinose, usually before the colonization of Ophiocordyceps sinensis owing to competitive inhibition and fruiting body formation. Therefore, monitoring C. farinosa in the O. sinensis cultivation environment is critical for minimizing the C. farinosa infection-induced losses. In this study, we initially designed a PCR primer pair (Tar-1F/Tar-1R) through open reading frame prediction and homology comparison of the C. farinosa genome sequence. This primer pair can detect both C. farinosa and Samsoniella hepiali. To further distinguish, primers (ITS5-172/ITS4-95) were then designed to selectively amplify the large ribosomal subunit sequences in the C. farinosa genome. All these primers were applied in combination for detection of C. farinosa in soil samples. The sensitivity reached a detection limit of 1 × 10 6 spores/g soil. In addition, these primers can detect the presence of C. farinosa in dead T. armoricanus larval samples. This newly established rapid detection method provides important information for C. farinosa control during O. sinensis cultivation.
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