生物膜
微生物学
抗生素
铜绿假单胞菌
磷霉素
生物
抗生素耐药性
妥布霉素
环丙沙星
哌拉西林
细菌
庆大霉素
遗传学
作者
Elif Arslan,Muhammed Kürşad COŞKUN,Şeymanur Çobanoğlu,Mehtap Hülya Aslan,Ayşenur Yazıcı
标识
DOI:10.1016/j.diagmicrobio.2023.115931
摘要
The aim of this study was to determine the effects of 4 antibiotics (tobramycin, fosfomycin, ciprofloxacin, and piperacillin/tazobactam) against Pseudomonas aeruginosa motility, biofilm formation, and biofilm resistance gene expression changes using different methods including microscopy, microdilution, crystal violet staining, and qRT-PCR. Although the antibiotics reduced swarming motility, they inhibited biofilm formation to a greater extent than the minimum inhibitory concentration (MIC) value. The qRT-PCR results showed that the antibiotics, other than fosfomycin, decreased the expression levels of the selected biofilm resistance genes (ndvB, tssC1, PA5033 and PA2070) in the biofilm structure compared to planktonic cells. Furthermore, it was found that there was an increase in the expression levels of biofilm resistance genes in the antibiotic application groups compared to the biofilm structure that was not treated with antibiotics. These results showed for the first time that the treatment of antibiotics at sub-MIC concentrations increases the expression levels of biofilm-specific resistance genes and contributes to resistance and motility.
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