材料科学
间充质干细胞
压电
氟化物
复合材料
细胞生物学
无机化学
生物
化学
作者
Liuyue Xu,Wenxiang Ren,Yaoying Long,B. S. Yang,Li Chen,Wenlan Chen,Si‐Yi Chen,Yulin Cao,Di Wu,Jiao Qu,Li He,Yali Yu,Anyuan Zhang,Shan Wang,Hongxiang Wang,Ting Chen,Guifen Fan,Qiubai Li,Zhichao Chen
标识
DOI:10.1021/acsami.4c12725
摘要
Regenerative therapies based on mesenchymal stem cells (MSCs) show promise in treating a wide range of disorders. However, the replicative senescence of MSCs during in vitro expansion poses a challenge to obtaining a substantial quantity of high-quality MSCs. In this investigation, a piezoelectric β-poly(vinylidene fluoride) film-based culture plate (β-CP) was developed with an antisenescence effect on cultured human umbilical cord-derived MSCs. Compared to traditional tissue culture plates (TCPs) and α-poly(vinylidene fluoride) film-based culture plates, the senescence markers of p21, p53, interleukin-6 and insulin-like growth factor-binding protein-7, stemness markers of OCT4 and NANOG, and telomere length of MSCs cultured on β-CPs were significantly improved. Additionally, MSCs at passage 18 cultured on β-CPs showed significantly better multipotency and pro-angiogenic capacities in vitro, and higher wound healing abilities in a mouse model. Mechanistically, β-CPs rejuvenated senescent MSCs by improving mitochondrial functions and mitigating oxidative and glycoxidative stresses. Overall, this study presents β-CPs as a promising approach for efficient and straightforward antisenescence expansion of MSCs while preserving their stemness, thereby holding great potential for large-scale production of MSCs for clinical application in cell therapies.
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