Isolation and typing of herpes simplex virus from clinical specimens collected at National Taiwan University Hospital, 1981-1990.

From 1981 to 1990, 10,167 clinical specimens were collected at National Taiwan University Hospital from the cases with suspected viral infections. Three hundred of herpes simplex virus (HSV) were isolated from these samples (isolation rate; 3.0%). One hundred and fifty-one strains were isolated from non-genital, and 149 strains from genital areas. HSV strains were typed by direct immunofluorescence staining technique. The type specific monoclonal antibodies were used in the test. Most of the HSV strains isolated from non-genital areas were type 1 (HSV-1) (147/151, 97.4%) whereas most of the strains from genital areas were type 2 (HSV-2) (140/149, 94.0%). All nine strains of HSV-1 isolated from genital areas were from female patients. The predominance of HSV-1 in non-genital area infections, and of HSV-2 in genital area infections, did not change during the ten years under review.