Electrochemical and Catalytic Properties of Liver Microsomes Adsorbed to Magnetic Nanoparticles

Subcellular liver fractions (microsomes) act as an ideal in-vitro system to study the pharmacokinetics, drug metabolism and drug-drug interactions of new drugs. Therefore, developing microsomes based biosensors and bioreactors have immense significance in green drug metabolites, stereoselective chemical compound synthesis and drug sensing. Magnetic nanoparticles (MNP) have unique advantages such as large surface area and high loading capacity for enzymes/proteins which can enhance the electrochemical properties such as heterogeneous electrons transfer kinetics and catalysis.[1] Also our prior studies have shown edge plane pyrolytic graphite electrodes to exhibit good electron transfer kinetics and film stability when immobilized with microsomes.[2] In the present study, our objective was to use amine functionalized MNP to adsorb complex drug metabolizing microsomes on edge plane pyrolytic graphite electrodes without the need to isolate and purify enzymes to develop volume efficient bioreactors. The electrochemical properties and electrocatalytic conversion of a drug into its metabolite for the constructed bioelectrode will be discussed. Acknowledgements: We are grateful for the financial support by the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number R15DK103386. [1] S. Krishnan, and C.Walgama, Anal. Chem. 2013 , 85 , 11420−11426. [2] C. Walgama, R. Nerimetla, N. F. Materer, D. Schildkraut, J. F. Elman, and S. Krishnan, Anal. Chem. http://dx.doi.org/10.1021/ac5044362