表面等离子共振
反应杯
材料科学
生物传感器
三乙氧基硅烷
基质(水族馆)
胶体金
纳米技术
检出限
等离子体子
表面改性
光电子学
纳米颗粒
化学
色谱法
光学
复合材料
物理化学
地质学
物理
海洋学
作者
Seo Yeong Oh,Nam Su Heo,Hoomin Lee,Sung-Chan Jang,Gyeongsik Ok,Youngjin Cho,Yun Suk Huh
标识
DOI:10.3389/fbioe.2019.00299
摘要
This research demonstrates the development of a transmission-mode localized surface plasmon resonance (LSPR) sensor chip using a cuvette cell system for the sensitive detection of a biomolecule marker such as C-reactive protein (CRP). In order to develop a highly sensitive LSPR sensor chip, plasmonically active gold nanoparticles (AuNPs) were decorated onto various transparent substrates in the form of a uniform, high-density single layer using a self-assembly process. The transparent substrate surface was modified with amine functional groups via (3-Aminopropyl)triethoxysilane (APTES) treatment, and the ligand concentration and temperature (0.5% APTES at 60°C) were then optimized to control the binding energy with AuNPs. The optimized plasmonically active strip was subsequently prepared by dipping the amine-functionalized substrate into AuNPs for 8 h. The optimized plasmonic strip functionalized with anti-CRP was transformed into a portable LSPR sensor chip by placing it inside a cuvette cell system, and its detection performance was evaluated using CRP as a model sample. The detection limit for CRP using our LSPR sensor chip was 0.01 μg/mL, and the detection dynamic range was 0.01-10 μg/mL with a %CV of <10%, thus confirming its selectivity and good reproducibility. These findings illustrate that the highly sensitive portable LSPR biosensor developed in this study is expected to be widely used in a diverse range of fields such as diagnosis, medical care, environmental monitoring, and food quality control.
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