Prime editor integrase systems boost targeted DNA insertion and beyond

Previously developed genome engineering tools cannot efficiently direct site-specific long DNA insertion. Built on the prime editing platform, two recent studies have reported integrase-mediated site-specific long DNA integration in the human genome. These prime editor integrase (PEI) systems will unleash many exciting applications in humans, animals, and plants. Previously developed genome engineering tools cannot efficiently direct site-specific long DNA insertion. Built on the prime editing platform, two recent studies have reported integrase-mediated site-specific long DNA integration in the human genome. These prime editor integrase (PEI) systems will unleash many exciting applications in humans, animals, and plants.