分离(微生物学)
分辨率(逻辑)
时间分辨率
图像分辨率
纳米技术
光学(聚焦)
化学
极限(数学)
衍射
生物系统
计算机科学
人工智能
物理
生物信息学
光学
数学分析
数学
生物
材料科学
作者
Yuxian Lu,Pinlong Zhao,Jiandong Feng
标识
DOI:10.1002/cjoc.202200764
摘要
Comprehensive Summary Super‐resolution microscopy (SRM) is a powerful imaging technique that overcomes the diffraction limit and allows imaging of the object structure in the nanoscale. However, SRM suffers from a tradeoff between spatial and temporal resolution, which prevents further exploration in scientific discoveries. In this review, we mainly focus on the development of improving spatiotemporal resolution of SRM, including 1) SRM based on physical and computational principles, 2) physical and computational factors affecting SRM, from which we conclude some strategies for developing new types of SRM, 3) the summary of the various types of SRM based on physical and computational principles, as well as, the analysis of the ordinary and developing SRM. Both SRMs based on physical principles and computational principles can be realized with spatial isolation and temporal isolation methods. We expect this review will offer some new ideas to improve the spatial and temporal resolution simultaneously, which may lead to more new discoveries in biology, chemistry, and materials science.
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