Multisignal Biosensors Based on Mn Paramagnetic Relaxation and Nanocatalysis for Norovirus Detection

化学 诺如病毒 磁弛豫 纳米技术 生物传感器 顺磁性 环境化学 病毒学 病毒 凝聚态物理 磁化 磁场 生物化学 物理 生物 量子力学 材料科学
作者
Lei Huang,Xue Zhang,Zefeng Mao,Shuyue Ren,Huanying Zhou,Baolin Liu,Zhixian Gao
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:96 (10): 4031-4038 被引量:3
标识
DOI:10.1021/acs.analchem.3c03950
摘要

A multisignal method for the sensitive detection of norovirus based on Mn paramagnetic relaxation and nanocatalysis was developed. This dual-modality sensing platform was based on the strong relaxation generated by cracked Au@MnO2 nanoparticles (NPs) and their intrinsic enzyme-like activity. Ascorbic acid rapidly cracked the MnO2 layer of Au@MnO2 NPs to release Mn(II), resulting in the relaxation modality being in a "switch-on" state. Under the optimal conditions, the relaxation modality exhibited a wide working range (6.02 × 103–3.01 × 107 copies/μL) and a limit of detection (LOD) of 2.29 × 103 copies/μL. Using 4,4′,4″,4″′-(porphine-5,10,15,20-tetrayl) tetrakis (benzenesulfonic acid) (tpps)-β-cyclodextrin (tpps-β-CD) as a T1 relaxation signal amplification reagent, a lower LOD was obtained. The colorimetric modality exploited the "peroxidase/oxidase-like" activity of Au@MnO2 NPs, which catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB, which exhibited a working range (6.02 × 104–6.02 × 106 copies/μL) and an LOD of 2.6 × 104 copies/μL. In addition, the rapid amplification reaction of recombinase polymerase enabled the detection of low norovirus levels in food samples and obtained a working range of 101–106 copies/mL and LOD of 101 copies/mL (relaxation modality). The accuracy of the sensor in the analysis of spiked samples was consistent with that of the real-time quantitative reverse transcription polymerase chain reaction, demonstrating the high accuracy and practical utility of the sensor.
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