化学
诺如病毒
磁弛豫
纳米技术
生物传感器
顺磁性
环境化学
病毒学
病毒
凝聚态物理
磁化
磁场
生物化学
物理
生物
量子力学
材料科学
作者
Lei Huang,Xue Zhang,Zefeng Mao,Shuyue Ren,Huanying Zhou,Baolin Liu,Zhixian Gao
标识
DOI:10.1021/acs.analchem.3c03950
摘要
A multisignal method for the sensitive detection of norovirus based on Mn paramagnetic relaxation and nanocatalysis was developed. This dual-modality sensing platform was based on the strong relaxation generated by cracked Au@MnO2 nanoparticles (NPs) and their intrinsic enzyme-like activity. Ascorbic acid rapidly cracked the MnO2 layer of Au@MnO2 NPs to release Mn(II), resulting in the relaxation modality being in a "switch-on" state. Under the optimal conditions, the relaxation modality exhibited a wide working range (6.02 × 103–3.01 × 107 copies/μL) and a limit of detection (LOD) of 2.29 × 103 copies/μL. Using 4,4′,4″,4″′-(porphine-5,10,15,20-tetrayl) tetrakis (benzenesulfonic acid) (tpps)-β-cyclodextrin (tpps-β-CD) as a T1 relaxation signal amplification reagent, a lower LOD was obtained. The colorimetric modality exploited the "peroxidase/oxidase-like" activity of Au@MnO2 NPs, which catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB, which exhibited a working range (6.02 × 104–6.02 × 106 copies/μL) and an LOD of 2.6 × 104 copies/μL. In addition, the rapid amplification reaction of recombinase polymerase enabled the detection of low norovirus levels in food samples and obtained a working range of 101–106 copies/mL and LOD of 101 copies/mL (relaxation modality). The accuracy of the sensor in the analysis of spiked samples was consistent with that of the real-time quantitative reverse transcription polymerase chain reaction, demonstrating the high accuracy and practical utility of the sensor.
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