A microfluidic platform integrating dynamic cell culture and dielectrophoretic manipulation for in situ assessment of endothelial cell mechanics

脐静脉 细胞生物学 剪应力 机械生物学 内皮干细胞 细胞培养 微流控 化学 生物医学工程 生物物理学 材料科学 纳米技术 体外 医学 生物 生物化学 复合材料 遗传学
作者
Hao Yang,Tao Chen,Yichong Hu,Fuzhou Niu,Xinyu Zheng,Haizhen Sun,Liang Cheng,Lining Sun
出处
期刊:Lab on a Chip [Royal Society of Chemistry]
卷期号:23 (16): 3581-3592 被引量:2
标识
DOI:10.1039/d3lc00363a
摘要

The function of vascular endothelial cells (ECs) within the complex vascular microenvironment is typically modulated by biochemical cues, cell-cell interactions, and fluid shear stress. These regulatory factors play a crucial role in determining cell mechanical properties, such as elastic and shear moduli, which are important parameters for assessing cell status. However, most studies on the measurement of cell mechanical properties have been conducted in vitro, which is labor-intensive and time-consuming. Notably, many physiological factors are lacking in Petri dish culture compared with in vivo conditions, leading to inaccurate results and poor clinical relevance. Herein, we developed a multi-layer microfluidic chip that integrates dynamic cell culture, manipulation and dielectrophoretic in situ measurement of mechanical properties. Furthermore, we numerically and experimentally simulated the vascular microenvironment to investigate the effects of flow rate and tumor necrosis factor-alpha (TNF-α) on the Young's modulus of human umbilical vein endothelial cells (HUVECs). Results showed that greater fluid shear stress results in increased Young's modulus of HUVECs, suggesting the importance of hemodynamics in modulating the biomechanics of ECs. In contrast, TNF-α, an inflammation inducer, dramatically decreased HUVEC stiffness, demonstrating an adverse impact on the vascular endothelium. Blebbistatin, a cytoskeleton disruptor, significantly reduced the Young's modulus of HUVECs. In summary, the proposed vascular-mimetic dynamic culture and monitoring approach enables the physiological development of ECs in organ-on-a-chip microsystems for accurately and efficiently studying hemodynamics and pharmacological mechanisms underlying cardiovascular diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
欣慰的星月完成签到,获得积分10
1秒前
FashionBoy应助潇湘雪月采纳,获得10
4秒前
传奇3应助leanne采纳,获得10
4秒前
大模型应助Heartlark采纳,获得10
6秒前
8秒前
moxi摩西完成签到,获得积分10
8秒前
卷卷完成签到,获得积分10
10秒前
时笙发布了新的文献求助10
11秒前
11秒前
pterion完成签到,获得积分10
11秒前
11秒前
13秒前
哒哒完成签到,获得积分10
15秒前
15秒前
循环发布了新的文献求助10
15秒前
幽默毛衣发布了新的文献求助10
18秒前
20秒前
循环完成签到,获得积分10
20秒前
leanne发布了新的文献求助10
20秒前
21秒前
量子星尘发布了新的文献求助10
21秒前
22秒前
22秒前
开灯人和关灯人完成签到,获得积分20
23秒前
Stardust发布了新的文献求助10
24秒前
25秒前
FashionBoy应助爱笑晓曼采纳,获得10
26秒前
张雯思发布了新的文献求助10
27秒前
Priority完成签到,获得积分10
28秒前
光热效应发布了新的文献求助30
28秒前
风之星给风之星的求助进行了留言
28秒前
28秒前
ASH发布了新的文献求助10
28秒前
OxO完成签到,获得积分10
28秒前
29秒前
搜集达人应助快乐一江采纳,获得10
29秒前
30秒前
leanne完成签到,获得积分20
31秒前
幽默毛衣完成签到,获得积分10
32秒前
晨曦完成签到,获得积分10
32秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989390
求助须知:如何正确求助?哪些是违规求助? 3531487
关于积分的说明 11254109
捐赠科研通 3270153
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809174