Dual Enzyme-Triggered In Situ Crosslinkable Gelatin Hydrogels for Artificial Cellular Microenvironments

Macromolecular BioscienceVolume 16, Issue 11 p. 1570-1576 Communication Dual Enzyme-Triggered In Situ Crosslinkable Gelatin Hydrogels for Artificial Cellular Microenvironments Bae Young Kim, Bae Young Kim Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaSearch for more papers by this authorYunki Lee, Yunki Lee Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaSearch for more papers by this authorJoo Young Son, Joo Young Son Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaSearch for more papers by this authorKyung Min Park, Kyung Min Park Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon, 22012 South KoreaSearch for more papers by this authorKi Dong Park, Corresponding Author Ki Dong Park kdp@ajou.ac.kr Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaE-mail: kdp@ajou.ac.krSearch for more papers by this author Bae Young Kim, Bae Young Kim Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaSearch for more papers by this authorYunki Lee, Yunki Lee Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaSearch for more papers by this authorJoo Young Son, Joo Young Son Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaSearch for more papers by this authorKyung Min Park, Kyung Min Park Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon, 22012 South KoreaSearch for more papers by this authorKi Dong Park, Corresponding Author Ki Dong Park kdp@ajou.ac.kr Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499 South KoreaE-mail: kdp@ajou.ac.krSearch for more papers by this author First published: 25 August 2016 https://doi.org/10.1002/mabi.201600312Citations: 22Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Horseradish peroxidase (HRP) and hydrogen peroxide (H2O2)-mediated crosslinking reaction has become an attractive method to create in situ forming hydrogels. While the crosslinking system has been widely utilized, there are certain issues require improvement to extend their biomedical applications, including creation of stiff hydrogels without compromising cytocompatibility due to initially high concentrations of H2O2. A gelatin-based hydrogels formed through a dual enzyme-mediated crosslinking reaction using HRP and glucose oxidase (GOx) as an H2O2-generating enzyme to gradually supply a radical source in HRP-mediated crosslinking reaction is reported. The physicochemical properties can be controlled by varying enzyme concentrations. Furthermore the hydrogel matrices provide 3D microenvironments for supporting the growth and spreading of human dermal fibroblasts with minimized cytotoxicity, despite the cells being encapsulated within stiff hydrogels. These hydrogels formed with HRP/GOx have great potential as artificial microenvironments for a wide range of biomedical applications. Citing Literature Volume16, Issue11November 2016Pages 1570-1576 RelatedInformation