EGTA公司
DNA甲基化
有丝分裂指数
DNA
螯合作用
细胞周期
分子生物学
生物
有丝分裂
甲基化
基因表达
生物化学
基因
钙
化学
细胞生物学
有机化学
作者
Caiyun Zhang,Wenshuo Shi,Keshi Ma,Hongjie Li,Feixiong Zhang
摘要
In this study, when germinated <em>Triticum aestivum</em> L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca<sup>2+</sup> within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca<sup>2+</sup> chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
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