Combinatorial effects of conception and governor vessel electroacupuncture and human umbilical cord blood-derived mesenchymal stem cells on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion

医学 电针 脐带 间充质干细胞 干细胞 缺血 病变 细胞凋亡 病理 再生(生物学) 麻醉 解剖 心脏病学 细胞生物学 针灸科 化学 替代医学 生物 生物化学
作者
Zhuoxin Yang,Chen Pengdian,Haibo Yu,Wenshu Luo,Min Pi,WU Yong-gang,Ling Wang,Fuxia Yang,Yanhua Gou
标识
DOI:10.1016/s0254-6272(14)60012-1
摘要

To investigate the combinatorial effects of conception and governor vessel electroacupuncture (EA) and human umbilical cord blood-derived mesenchymal stem cells (HUCB-MSCs) on pathomorphologic lesion and cellular apoptosis in rats with cerebral ischemia/reperfusion. With the HUCB-MSCs isolated, cultured and identified and the models of cerebral ischemia-reperfusion established, the HUCB-MSCs of passage three were intracranially transplanted and the EA at conception and governor vessels was applied. The pathomorphologic lesion by hematoxylin-eosin staining and the cellular apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling method around the ischemic focus were observed. The cultured adherent HUCB-MSCs exhibited a spindle shape and expressed MSC-specific markers, with the cell purity and proliferation rate significantly increasing after the primary passage. HE staining showed that there were no pathological changes observed in the sham surgery group. However, in the PBS transplantation group, degeneration and necrosis of a great number of nerve cells were seen. In both the HUCB-MSCs transplantation group and the HUCB-MSCs transplantation + EA group, reparative changes of the pathomorphism of the tissue were found. Both combination treatment and simple MSCs treatment were able to improve the pathomorphorlogic lesion following cerebral ischemia and reduce the abnormal TUNEL-positive numbers, with former better than latter. HUCB-MSCs improve pathological lesions and inhibit the cellular apoptosis around the cerebral ischemic area. EA at conception and governor vessels also improve pathological lesion and inhibit the cellular apoptosis in rats treated with HUCB-MSCs transplantation, which effects were superior to that of simple HUCB-MSCs transplantation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
sln完成签到,获得积分10
刚刚
Jerry完成签到,获得积分10
刚刚
为你等候完成签到,获得积分10
刚刚
Bioflying完成签到,获得积分10
8秒前
五月完成签到 ,获得积分10
9秒前
欢呼曼荷完成签到,获得积分10
12秒前
端庄洪纲完成签到 ,获得积分10
13秒前
14秒前
洋洋爱吃枣完成签到 ,获得积分10
19秒前
开心果发布了新的文献求助10
19秒前
嘻嘻完成签到,获得积分10
21秒前
22秒前
义气的身影完成签到,获得积分10
22秒前
mf2002mf完成签到 ,获得积分10
24秒前
开心果完成签到,获得积分10
30秒前
米里迷路完成签到 ,获得积分10
31秒前
乘舟江行完成签到,获得积分10
31秒前
科研通AI2S应助白华苍松采纳,获得10
32秒前
神行太保完成签到,获得积分10
33秒前
zxp发布了新的文献求助10
36秒前
xiaoluuu完成签到 ,获得积分10
37秒前
spy完成签到 ,获得积分10
39秒前
坦率绮山完成签到 ,获得积分10
40秒前
wenhuanwenxian完成签到 ,获得积分10
43秒前
kehe!完成签到 ,获得积分0
48秒前
心随以动完成签到 ,获得积分10
49秒前
maxyer完成签到,获得积分10
52秒前
zdu完成签到,获得积分10
53秒前
修辛完成签到 ,获得积分10
56秒前
橘子小西完成签到 ,获得积分10
57秒前
小九完成签到,获得积分10
59秒前
课呢完成签到,获得积分10
1分钟前
Much完成签到 ,获得积分10
1分钟前
刘蓬勃完成签到 ,获得积分10
1分钟前
窝窝头完成签到 ,获得积分10
1分钟前
1分钟前
嘿嘿完成签到 ,获得积分10
1分钟前
多边形完成签到 ,获得积分10
1分钟前
孙某人完成签到 ,获得积分10
1分钟前
刘蓬勃发布了新的文献求助10
1分钟前
高分求助中
The late Devonian Standard Conodont Zonation 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3244791
求助须知:如何正确求助?哪些是违规求助? 2888434
关于积分的说明 8252939
捐赠科研通 2556941
什么是DOI,文献DOI怎么找? 1385522
科研通“疑难数据库(出版商)”最低求助积分说明 650176
邀请新用户注册赠送积分活动 626303